Search results for "Germplasm conservation"

Morphological and molecular variability within the fig cultivar 'Dottato' in the Italian protected designation origin area "fichi di Cosenza"

2017

The morphological and molecular diversity among fig accessions of 'Dottato', found in the PDO "Fichi di Cosenza" area was studied by evaluating 24 morphological traits and by genotyping with 18 microsatellite markers. The microsatellite allelic profiles among the putative clones of 'Dottato' indicated a moderate genetic variability, discriminating unambiguously most of the accessions. Only two groups of identity were found. The average expected and observed heterozygosity were 0.43 and 0.62, respectively. The mean polymorphic information content (PIC) was 0.4, varying from 0.08 (LMFC26) to 0.067 (FCUP 38-6). The morphological clustering allowed the distinction of all genotypes. Some genotyp…

Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm

2022

The characterization of plant genetic resources is a precondition for genetic improvement and germplasm management. The increasing use of molecular markers for DNA-based genotype signature is crucial for variety identification and traceability in the food supply chain. We collected 75 Sicilian hazelnut accessions from private and public field collections, including widely grown varieties from the Nebrodi Mountains in north east Sicily (Italy). The germplasm was fingerprinted through nine standardized microsatellites (SSR) for hazelnut identification to evaluate the genetic diversity of the collected accessions, validating SSR discrimination power. We identified cases of homonymy and synonym…

Seed cryopreservation of Fraxinus angustifolia Vahl

2016

Fraxinus angustifolia is used for afforestation and for production of both valuable timber and manna , a substance with pharmacological applications. The aim of this research was to establish the optimized condition for cryopreservation of F. angustifolia seeds, in order to protect the germplasm of this species. Germination percentage and mean time of germination of non-treated seeds (control) and liquid nitrogen-treated seeds were established in water or in 10 -6 M gibberellic acid (GA 3 ). The seeds could be cryopreserved with 3% of moisture content (MC) and germinated easily in water (70.0 ± 5.0%), while seeds cryopreserved with 6% MC showed a physiological dormancy. This state could be …

Assessment of genetic variation in SicilianHelichrysum(Asteraceae) and implication to germplasm conservation

2008

Some taxa of Sicilian Helichrysum related to sect. Stoeachadina have an uncertain systematic position; moreover, they are rare and endangered. In order to preserve these taxa in a genebank, a strategy to keep their genetic variation as high as possible is required. The high informative content of AFLP markers was used to assess genetic variations within and between populations and their relationships. The results show that all populations are genetically similar, even though there are two distinct groups: the first is the population referred to H. pendulum, and the second is represented by other populations belonging to several different specific and intraspecific taxa. The H. pendulum popu…

APPROACHES TO STUDY BIODIVERSITY AND SALINITY TOLERANCE MECHANISMS IN MEDITERRANEAN FRUIT TREE SPECIES

Changes in global climate are going to increase the problem of drought and soil salinity in the Mediterranean area, where increases in temperature, drought and soil and water salinity are expected. Identification of plant genotypes with positive agronomic traits, such as salt tolerance, may reduce the effects of salinity and drought on productivity. Therefore, there is considerable effort being directed toward the development of salt-tolerant genotypes through plant breeding, with the aim to the introgression of salt-tolerance traits into new cultivars. The results presented in this thesis contributed to the develop of new tools to improve knowledge on the biodiversity and salinity toleranc…

Seed germination in Abies nebrodensis (Pinaceae)

2019

Abies nebrodensis is a strict Sicilian endemic species, listed as “Critically Endangered” in the IUCN Red List of Threatened Species. Seed production highly varies annually and among specimens. The species is affected by a very low seed germination rate, mainly depending on the large number of empty seeds (from 68 to 96%). The embryo presence in seeds increases with increasing seed size and weight. Optimal germination rate (61%) was achieved at constant 5°C in the darkness. The low proportion of embryos in seeds is a relevant threat for A. nebrodensis conservation, as it affects the intrapopulation genetic diversity and reduces the potential genetic variability of seed accessions preserved …

THE BOTANICAL GARDEN “BERNARDINO DA UCRIA” IN THE NATURAL PARK OF THE NEBRODI (SICILY) AND ITS MISSION TO CONSERVE, EXPLOIT AND SPREAD LOCAL AGROBIOD…

2016

In Sicily, the academic botanical gardens of Catania, Messina and Palermo have been historically exerting a multiplicity of activities ranging from maintenance of ex situ collections to plant conservation policy, practice and ecological restoration, along with more traditional functions related to education and academic research. In the last decade’s, two new botanical gardens the “Nuova Gussonea” and the “Bernardino da Ucria, were created in Sicily, with the aim to play more delimited, yet modern and complementary roles. The garden “Nuova Gussonea”, within the Etna Natural Park, is mostly devoted to collect and preserve the native flora of the Mount Etna, with a special focus on endemic an…

Ecophysiology of germination in Sicilian population of Ferula communis (Umbelliferae)

2010

Ferula communis L. is an umbelliferous plant widespread in the Mediterranean basin and particularly abundant in Sicily and Sardinia islands (Italy). In this species, two chemotypes have been distinguished: one is poisonous and responsible of hemorrhagic syndrome of livestock, known as ferulosis, while the other one (non- poisonous) has anticancer activity due to the presence of daucane esters. In Sicily the dry stem of this plant has commercial applications in special manufactured products. The object of the research was to investigate the germination ecophysiology of nine Sicilian populations of F. communis, in order to establish the correct germplasm conservation of the chemotypes economi…